Current Issue : January-March Volume : 2016 Issue Number : 1 Articles : 6 Articles
A simple gas chromatographic method has been developed for the determination of methyl methane sulfonate, ethyl methane sulfonate and isopropyl methane sulfonate in emtricitabine drug substance. The method was validated as per ICH guideline in terms of LOD, LOQ, method precision, accuracy and specificity. The LOD and LOQ values were found to be 0.5 ppm (0.5 µg/g) and 1.5 ppm (1.5 µm/g) respectively....
Reverse phase high performance liquid chromatographic (RPHPLC) method was developed for the simultaneous estimation of naltrexone and bupropion in combined dosage form. The separation was achieved by C18 Column (250 x 4.6 mm, 5 µm Particle Size) and the mobile phase consisting of 0.05 M potassium dihydrogen phosphate (pH adjusted to 4.5±0.1): methanol (30:70 (V/V)). Detection was carried out at 224 nm. Retention time of naltrexone and bupropion was found to be 6.81 minutes and 4.26 minutes respectively. This method has been validated for linearity, accuracy and precision. Linearity for naltrexone was found in range of 2 to 6 mcg/ml and bupropion was found in range of 22.5 to 67.5mcg/ml. The coefficient correlation of naltrexone and bupropion was found to be 0.9991 and 0.9986 respectively. The percentage recoveries for naltrexone and bupropion were found to be in range of 99.52 to 100.31% and 100.11 to 100.70% respectively. In this method the first order derivative of UV spectrometry method was found to be simple, accurate, fast, precise and reproducible. The first derivative values measured at 210 nm for naltrexone and 264 nm for bupropion. The linearity for naltrexone and bupropion were found in the range of 2 to 6 mcg/ml and 22.5 to 67.5 mcg/ml respectively. The coefficient correlation of naltrexone and bupropion was found to be 0.9998 and 0.9994 respectively. The percentage recoveries obtained for naltrexone and bupropion were found to be in range of 99.38 to 99.92% and 99.26 to 99.94% respectively. Developed method was found to be accurate, precise and rapid for simultaneous estimation of naltrexone and bupropion....
A simple, accurate and precise dual wavelength spectrophotometric method was developed for simultaneous determination of amiloride hydrochloride and furosemide in combined pharmaceutical dosage form. The principle for dual wavelength method is “the absorbance difference between two points on the mixture spectra is directly proportional to the concentration of the component of interest”. The wavelengths selected for determination of amiloride hydrochloride were 281 nm and 271 nm, whereas, the wavelengths selected for determination of furosemide were 280 nm and 290 nm. Methanol was taken as a solvent. Regression analysis of Beer’s plots showed good correlation in concentration range of 2-10 μg/ml for amiloride hydrochloride and 16-80 μg/ml for furosemide. Accuracy of method was found within limits. The precision (intra‐day, inter‐day and repeatability) of method was found within limits. The methods were validated according to ICH guidelines for evaluation of accuracy, LOD and LOQ, precision, repeatability, reproducibility etc. The proposed method was successfully applied to determination of these drugs in commercial tablets....
Quercetin is a flavonoid and is a major constituent of the species Ipomoea sepiaria koenig ex. Roxb. Analytical thin layer chromatography was carried out on silica gel GF254 TLC plates and the chromatoplate was developed with chloroform:acetone:formic acid (76:16.5:85 v/v) as mobile phase. An RP-HPLC method was developed for the estimation of quercetin from of Ipomoea sepiarea. Mobile phase composed of acetonitrile: methanol: triethyle amine (45:55:0.5% v/v). The flow rate was 1.2 ml/min and the analyses of column effluents were performed using uv-visible detector at 264 nm. Retention time of quercetin was found as 1.5 min. This method has obeyed linearity over the concentration range of 2-10 µg/ml and the regression coefficient obtained from linearity plot for quercetin was found as 0.999. RP-HPLC method was validated in pursuance of ICH guidelines....
Gomutra Haritaki is one of the Ayurvedic Drug which has been indicated for Granthi, Arbuda, Apachi, Pandu, Sotha, Arsha. Here to treat Garbhashaya Arbuda (Uterine fibroid) Gomutra Haritaki was taken. The present work was carried out to standardize the finished product Gomutra Haritaki to conform its identity, quality and purity. The pharmacognostical work reveals that presence of simple and compound grains, vessels with simple pits and groups of sclereids, few fibres, stone cells, mesocarp cells and tannin containing cells had observed microscopically from fruit of Terminalia chebula. Organoleptic features of GH made out of the crude drugs were within the standard range as mentioned in the classic. The pH value of GH was 4.5, Loss on drying was 5.05 %w/w, ash value was 6.51 %w/w, water-soluble extract was 59.69 %w/w, methanol soluble extraction was 59.27 %w/w, acid insoluble ash value was 2.43% w/w and high performance thin layer chromatography (HPTLC) at 254 nm and 366 nm resulted into 8 and 7 spots respectively....
Ipomoea sepiaria Koenig Ex. Roxb (convolvulaceae) is otherwise called as hedge bindweed. It is climber distributed in tropical and subtropical region. Ethanomedical and traditional usage of this plant reveals antidote for arsenic poisoning, uterine tonic, aphrodisiac and antiulcer, diuretic, leucorrhoea and in burning sensation during urination. Till date the plant was not explored about phytochemical, physicochemical and phytopharmacological relevance. Hence we aimed to study the physicochemical and preliminary phytochemical relevence. Separation of quercetin was attempted. The separated quercetin was characterized by 1H NMR and MASS spectrophotometry. This study paved the presence of quercetin in methanol and acetone extracts of Ipomoea sepiaria koenig ex. Roxb....
Loading....